Global Light Sheet Microscope Market Research Report 2023-Competitive Analysis, Status and Outlook by Type, Downstream Industry, and Geography, Forecast to 2029

Global Light Sheet Microscope Market Research Report 2023-Competitive Analysis, Status and Outlook by Type, Downstream Industry, and Geography, Forecast to 2029

Light sheet microscopy, often referred to as single plane illumination microscopy (SPIM), is a rapidly emerging technology that combines optical sectioning with multiple-view imaging to observe tissues and living organisms with impressive resolution. Unlike the conventional techniques of widefield and confocal fluorescence microscopy, the light sheet technique illuminates on the region surrounding the focal plane of the detection objective in a twin objective configuration (where the objectives are juxtaposed at 45-degrees). When compared to conventional microscopy, light sheet methods exhibit reduced photobleaching and lower phototoxicity, and often enable far more scans per specimen. By rotating the specimen, the technique can image virtually any plane with multiple views obtained from different angles. Thus, light sheet microscopy is ideal for examining of both large (animals) and small (cells) specimens labeled with fluorescent proteins and other fluorophores.

Market Overview:

The latest research study on the global Light Sheet Microscope market finds that the global Light Sheet Microscope market reached a value of USD 137.82 million in 2022. It’s expected that the market will achieve USD 228.7 million by 2028, exhibiting a CAGR of 8.81% during the forecast period.

COVID-19 is generally considered as a respiratory disease. SARS-CoV-2 virus, which caused COVID-19 pandemic, is a single-stranded RNA virus. The virus is thought to be transmitted from animals. However, SARS-CoV-2 also affects many organs including the central nervous system.

Detection and imaging of SARS-CoV-2 in tissues are mainly accomplished by traditional immunohistochemical methods. However, because these thin slices are only based on tissues, they cannot provide a complete spatial image of infection.

Advances in tissue optical transparency (TOC) methods have made large and complete tissues optically transparent. This eliminates the need for physical slicing and uses optical slicing to provide 3D images.

Previous studies have reported 3D imaging of lung tissue infected with SARS-CoV-2. One study used phase contrast tomography to study unstained lung tissue. There are also reports of fluorescent H&E simulated staining to obtain 3D pseudo-histological imaging.

However, the emerging method of high-resolution, large-scale and multi-scale imaging of tissue microstructure using fluorescence labeling and tissue clearance can obtain 3-dimensional histology, which can open up new insights for SARS-Cov-2 infection and the nature of COVID -19 disease process.

With the successful 3D visualization of SARS-CoV-2 infection, this study can be used as proof of concept for studying important respiratory pathogens in intact tissues and organs. The light sheet microscopy provides the first choice for researchers seeking large-scale and high-resolution 3D fluorescence imaging to analyze and study COVID-19 pathology.

Market Drivers

In the 17th century, the optical microscope invented by Dutch scientist Leeuwenhoek presented the mysterious, complex and colorful microscopic world to human beings. Optical microscope has become an indispensable tool for biomedical research because of its advantages of low damage to samples, non-invasion and abundant imaging mechanism. Optical microscopic imaging technology has directly promoted the rapid development of cytology, histology, embryology, microbiology and paleontology, so that human beings can explore the evolution of life, understand the principle of diseases and decode the secrets of genes. The continuous innovation of optical microscopic imaging technology has brought new discoveries and breakthroughs to biomedical research, and the development of biomedicine has in turn put forward higher requirements for the imaging speed, spatial resolution, information acquisition dimension and imaging quality of optical microscopy.
The requirements of biological optical microscope are very high, and more and more complex research applications need instruments that can keep up with the pace. Over the years, biological imaging has been developing in the direction of using more and more physiological related systems for experiments, and new technologies have made this experimental method more and more feasible. Light-induced photodamage and phototoxicity have long been a problem in the field of biological imaging, and can have a considerable impact on the health and function of tissues at all biological levels. Therefore, imaging large and sensitive samples requires an effective 3D imaging method to minimize the exposure of samples to light.

Market Challenges
and Restraints
The influence of light sheet microscopy can be seen in various fields such as development and cell biology, anatomy, biophysics and neuroscience. Although its performance is usually superior and its adoption by biologists has been stable, light sheet microscopy has not replaced more traditional imaging methods. One reason for this is that the field has largely conformed to a do-it-yourself ethic, the powerful implementation of Although the Challenges of Big Image Data Can't Be Over Stated. Light sheet Microscopy is only applicable to minorities all over the world, so the scope of this technology is unnecessarily limited.
Although the birth of light sheet microscopy has greatly expanded the research field of life science, it also has some inherent defects and technical difficulties that need to be overcome.
The imaging performance of light sheet microscopy is closely related to the characteristics of the optical sheet. The axial resolution of the system is determined by the numerical aperture of the detection objective and the thickness of the laser sheet, and the FOV is limited by the width of the optical sheet. Traditional optical film is realized by Gaussian beam passing through a cylindrical lens. Initially, only one illumination objective and one imaging objective were used. The biggest problem of this kind of optical film is uneven illumination, and the light intensity will gradually decrease along the propagation direction. In order to solve this problem, the effective field of view can only choose a relatively uniform illumination range, so the sample must be rotated at least four times by 90. In this way, the imaging speed is very slow, and complex algorithms are used for post-processing, which is easy to produce artifacts.

Region Overview:

In 2022, the share of the Light Sheet Microscope market in Europe stood at 42.92.%.

Company Overview:

Leica is one of the major players operating in the Light Sheet Microscope market, holding a share of 31.03% in 2023.

Leica

Leica Microsystems develops and manufactures microscopes and scientific instruments for the analysis of microstructures and nanostructures. Ever since the company started as a family business in the nineteenth century, its instruments have been widely recognized for their optical precision and innovative technology. It is one of the market leaders in compound and stereo microscopy, digital microscopy, confocal laser scanning microscopy with related imaging systems, electron microscopy sample preparation, and surgical microscopes.

Zeiss

ZEISS is one of the world leading manufacturers of microscopes. In addition to excellent light and electron/ion microscopes, ZEISS also offers a broad range of optical sectioning systems as well as high-resolution X-ray microscopes. ZEISS Microscopy is a leading provider of microscope solutions in the life sciences, materials research, routine and industry markets.

Segmentation Overview:

By type, SPIM segment accounted for the largest share of market in 2022.

SPIM

Selective Plane Illumination Microscopy (SPIM) is fast and gentle imaging technique that combines the speed of widefield imaging with moderate optical sectioning and low photobleaching. It has become an important fluorescence imaging modality, especially for volumetric imaging.
The main disadvantage of SPIM is that extra optics are required to generate the light sheet. Most commonly a separate illumination objective lens is placed orthogonally to the detection objective and sheet-generating optics are placed between this illumination objective and the laser source. Adding the extra lens introduces steric constraints to both the imaging system and sample mounting. In essence, the microscope needs to be designed around the sample, so there are a wide variety of light sheet microscope designs each optimal for different samples and different mounting requirements. In contrast, traditional confocal or epi-fluorescent microscopes have a single optical path and can accommodate a much wider variety of samples. In other words, the advantages of SPIM come at the cost of more narrow applicability of any single instrument; this has led to the explosion of different light sheet microscope configurations, each with their own advantages and disadvantages.

DiSPIM

The DISPIM is a dual light sheet microscope that acquires two orthogonal views of a sample. These two views can then be fused and joint deconvolved to generate a single volume with high isotropic resolution. Like other light sheet microscopes, the DISPIM provides rapid volume imaging with a low photodose and is therefore ideally suited for long term or high repetition rate imaging in samples where phototoxicity/photobleaching can be a problem. Unlike conventional light sheet microscopes, the joint deconvolution process provides good resolution in all directions.

Application Overview:

The market's largest segment by application is the segment Developmental Biology, with a market share of 52.75% in 2022.

Key Companies in the global Light Sheet Microscope market covered in Chapter 3:

Intelligent Imaging Innovations
Bruker
Leica
Olympus Corporation
Miltenyi Biotec
Zeiss
ASI

In Chapter 4 and Chapter 14.2, on the basis of types, the Light Sheet Microscope market from 2018 to 2029 is primarily split into:

SPIM
DiSPIM
Others

In Chapter 5 and Chapter 14.3, on the basis of Downstream Industry, the Light Sheet Microscope market from 2018 to 2029 covers:

Developmental Biology
Nephrology
Neuronal Morphology
Immunology
Others

Geographically, the detailed analysis of consumption, revenue, market share and growth rate, historic and forecast (2018-2029) of the following regions are covered in Chapter 8 to Chapter 14:

North America (United States, Canada)
Europe (Germany, UK, France, Italy, Spain, Russia, Netherlands, Turkey, Switzerland, Sweden)
Asia Pacific (China, Japan, South Korea, Australia, India, Indonesia, Philippines, Malaysia)
Latin America (Brazil, Mexico, Argentina)
Middle East & Africa (Saudi Arabia, UAE, Egypt, South Africa)